Unknown

Dataset Information

0

Non-coding cis-element of Period2 is essential for maintaining organismal circadian behaviour and body temperature rhythmicity.


ABSTRACT: Non-coding cis-regulatory elements are essential determinants of development, but their exact impacts on behavior and physiology in adults remain elusive. Cis-element-based transcriptional regulation is believed to be crucial for generating circadian rhythms in behavior and physiology. However, genetic evidence supporting this model is based on mutations in the protein-coding sequences of clock genes. Here, we report generation of mutant mice carrying a mutation only at the E'-box cis-element in the promoter region of the core clock gene Per2. The Per2 E'-box mutation abolishes sustainable molecular clock oscillations and renders circadian locomotor activity and body temperature rhythms unstable. Without the E'-box, Per2 messenger RNA and protein expression remain at mid-to-high levels. Our work delineates the Per2 E'-box as a critical nodal element for keeping sustainable cell-autonomous circadian oscillation and reveals the extent of the impact of the non-coding cis-element in daily maintenance of animal locomotor activity and body temperature rhythmicity.

SUBMITTER: Doi M 

PROVIDER: S-EPMC6561950 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC2242825 | biostudies-literature
| S-EPMC2695588 | biostudies-literature
| S-EPMC10927146 | biostudies-literature
| S-EPMC3798035 | biostudies-literature
| S-EPMC6611576 | biostudies-literature
| S-EPMC5651750 | biostudies-literature
| S-EPMC3173173 | biostudies-literature
| S-EPMC3405291 | biostudies-other
| S-EPMC7332991 | biostudies-literature
| S-EPMC3695510 | biostudies-literature