Dataset Information

In vitro sensitivity of malignant melanoma cells lines to photon and heavy ion radiation.

ABSTRACT:

Background

The role of radiotherapy in malignant melanoma is still in discussion due to its relative resistance to radiation. In various literature, heavy ions show a higher relative biological effectiveness than photons. The aim of this work is to evaluate the radiotherapeutical effect from photons as well as heavy ions on malignant melanoma cells and to indicate the possible radiosensitivity based on its proliferation-inhibitory effect.

Methods

Two different cell lines of malignant melanoma, WM115 (primary tumor) and WM266-4 (metastatic site, skin) were used in this in vitro study. The cells were treated with photons or heavy ions (C₁₂ and O₁₆ ions). Cell-proliferation assay using hemocytometer was used for the quantitative and qualitative evaluation of cell growth. Furthermore, flow cytometry was also used to analyze the cell cycle distribution.

Results

Heavy ions compared to photons and between the two heavy ion modalities, O₁₆ ions showed an improved suppression of cell growth in both cell lines. Furthermore, a G2/M arrest was detected in both cell lines after all radiotherapy modalities - with the arrest increasing with the dose applied.

Conclusion

Heavy ions showed a greater inhibitory effect on cell proliferation compared to photons and an increased G2/M arrest. Therefore, C₁₂ and O₁₆ heavy ions might overcome the relative radioresistance of malignant melanoma to photons. Further research is warranted.

SUBMITTER: Aninditha KP

PROVIDER: S-EPMC6562297 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature

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Publications

In vitro sensitivity of malignant melanoma cells lines to photon and heavy ion radiation.

Aninditha K P KP Weber K J KJ Brons S S Debus J J Hauswald H H

Clinical and translational radiation oncology 20190608

<h4>Background</h4>The role of radiotherapy in malignant melanoma is still in discussion due to its relative resistance to radiation. In various literature, heavy ions show a higher relative biological effectiveness than photons. The aim of this work is to evaluate the radiotherapeutical effect from photons as well as heavy ions on malignant melanoma cells and to indicate the possible radiosensitivity based on its proliferation-inhibitory effect.<h4>Methods</h4>Two different cell lines of malign ...[more]

PMID: 31211251

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Project description:BackgroundThe objective of this study was to evaluate the outcomes of patients with high-grade glioma who received treatment with particle radiotherapy.MethodsBetween June 2015 and October 2018, 50 consecutive and nonselected patients with glioblastoma multiforme (n = 34) or anaplastic glioma (n = 16) were treated at the Shanghai Proton and Heavy Ion Center. Twenty-four patients received proton radiotherapy (at a dose of 60 gray-equivalents in 30 daily fractions), and 26 patients received proton radiotherapy plus a carbon-ion radiotherapy (CIRT) boost in various dose-escalating schemes. All patients received temozolomide because of their age or their O-6-methylguanine-DNA methyltransferase (MGMT) promoter methylation status. Progression-free survival (PFS) and overall survival (OS) rates, as well as treatment-induced toxicities, were analyzed.ResultsAt a median follow-up of 14.3 months (range, 4.8-39.6 months), the 12-month and 18-month OS rates were 87.8% (95% CI, 77.6%-98.0%) and 72.8% (95% CI, 56.7%-88.9%), respectively, and the 12-month and 18-month PFS rates were 74.2% (95% CI, 60.9%-87.5%) and 59.8% (95% CI, 43.1%-76.5%), respectively. Univariate analyses revealed that age (>50 vs ≤50 years), World Health Organization grade (3 vs 4), and Karnofsky performance status (>80 vs ≤80) were significant prognosticators for OS, and IDH mutation and World Health Organization grade were significant for predicting PFS. Furthermore, MGMT promoter methylation, performance status, and age showed a trend toward predicting PFS. No significant predictive factors for PFS or OS were identified in multivariate analyses. Twenty-nine patients experienced grade 1 treatment-related acute adverse effects, and 11 developed grade 1 (n = 6) or grade 2 (n = 5) late adverse effect of radiation-induced brain necrosis. No grade 3, 4, or 5 toxicities were observed.ConclusionsParticle radiotherapy produced 18-month OS and PFS rates of 72.8% and 59.8%, respectively, with acceptable adverse effects in patients with high-grade glioma. Particle radiotherapy at a dose ≥60 gray-equivalents appears to be safe and potentially effective.

Dataset Information

In vitro sensitivity of malignant melanoma cells lines to photon and heavy ion radiation.

Background

Methods

Results

Conclusion

Publications

In vitro sensitivity of malignant melanoma cells lines to photon and heavy ion radiation.

Similar Datasets

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