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Molecular Characterization of Sarcocystis Species Isolated from Sheep and Goats in Riyadh, Saudi Arabia.

ABSTRACT: Sarcocystosis is induced by species of Sarcocystis, which is an intracellular protozoan parasite in the phylum Apicomplexa. The diversity and importance of Sarcocystis species in sheep and goats in Saudi Arabia are poorly understood. In this study, the tongue, esophagus, heart, diaphragm, and skeletal muscles were collected from 230 sheep and 84 goats, and the tissues were examined for the presence of Sarcocystis species by macroscopic examination and light microscopy. Microscopic Sarcocystis species cysts were found in both sheep and goats. Transmission electron microscopy (TEM) revealed S. tenella in sheep and S. capracanis in goats. Sarcocystis species were confirmed for the first time in Saudi Arabian sheep and goats by molecular testing. S. capracanis was most closely related to S. tenella, with the COX1 sequences sharing 91.7% identity. A phylogenetic analysis produced similar results and indicated that the Sarcocystis isolates were within a group of Sarcocystis species in which dogs were the final host. Finally, the Sarcocystis species cysts from sheep and goats could be grouped together, indicating that they were strongly related.

SUBMITTER: Metwally DM

PROVIDER: S-EPMC6562545 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature

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Project description:BackgroundMicrobial biodegradation of oil-hydrocarbons is one of the sustainable and cost-effective methods to remove petroleum spills from contaminated environments. The current study aimed to investigate the biodegradation abilities of three Fusarium isolates from oil reservoirs in Saudi Arabia. The novelty of the current work is that the biodegradation ability of these isolates was never tested against some natural hydrocarbons of variable compositions, such as Crude oil, and those of known components such as kerosene and diesel oils.MethodsThe isolates were treated with five selected hydrocarbons. The hydrocarbon tolerance test in solid and liquid media was performed. The scanning electron microscope (SEM) investigated the morphological changes of treated fungi. 2, 6-Dichlorophenol Indophenol (DCPIP), drop collapse, emulsification activity, and oil Spreading assays investigated the biodegradation ability. The amount of produced biosurfactants was measured, and their safety profile was estimated by the germination assay of tomato seeds.ResultsThe tolerance test showed enhanced fungal growth of all isolates, whereas the highest dose inhibition response (DIR) was 77% for Fusarium proliferatum treated with the used oil (p < 0.05). SEM showed morphological changes in all isolates. DCPIP results showed that used oil had the highest biodegradation by Fusarium verticillioides and Fusarium oxysporum. Mixed oil induced the highest effect in oil spreading, drop collapse, and emulsification assay caused by F. proliferatum. The highest recovery of biosurfactants was obtained by the solvent extraction method for F. verticillioides (4.6 g/L), F. proliferatum (4.22 g/L), and F. oxysporum (3.73 g/L). The biosurfactants produced by the three isolates stimulated tomato seeds' germination more than in control experiments.ConclusionThe current study suggested the possible oil-biodegradation activities induced by three Fusarium isolates from Riyadh, Saudi Arabia. The produced biosurfactants are not toxic against tomato seed germination, emphasizing their environmental sustainability. Further studies are required to investigate the mechanism of biodegradation activities and the chemical composition of the biosurfactants produced by these species.

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Molecular Characterization of Sarcocystis Species Isolated from Sheep and Goats in Riyadh, Saudi Arabia.

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