Unknown

Dataset Information

0

Feasibility of measuring sodium, potassium and creatinine from urine sample on dried filter paper.


ABSTRACT: Aim: To develop a bioassay for estimation of sodium, potassium and creatinine in dried urine strips and comparing with their respective concentration in liquid urine samples. Materials & methods: Urine was collected on filter paper strips, dried at room temperature and, eluted for estimation of sodium, potassium by indirect ion selective electrode method and creatinine by Jaffé method. Result: This bioassay was validated based on the US FDA guidelines for bioanalytical method validation and was linear, sensitive, accurate and precise with acceptable recovery and matrix effects. Analytes were stable in dried urine strips during 1 year of storage at 4°C. Conclusion: We conclude that the dried urine is suitable for analysis of sodium, potassium and creatinine and offers a convenient alternative for monitoring dietary salt intake.

SUBMITTER: Tarik M 

PROVIDER: S-EPMC6562700 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature

altmetric image

Publications

Feasibility of measuring sodium, potassium and creatinine from urine sample on dried filter paper.

Tarik Mohamad M   Ramakrishnan Lakshmy L   Amarchand Ritvik R   Salve Harshal Ramesh HR   Mathur Prashant P   Joshi Pradeep P   Krishnan Anand A  

Bioanalysis 20190417 8


<b>Aim:</b> To develop a bioassay for estimation of sodium, potassium and creatinine in dried urine strips and comparing with their respective concentration in liquid urine samples. <b>Materials & methods:</b> Urine was collected on filter paper strips, dried at room temperature and, eluted for estimation of sodium, potassium by indirect ion selective electrode method and creatinine by Jaffé method. <b>Result:</b> This bioassay was validated based on the US FDA guidelines for bioanalytical metho  ...[more]

Similar Datasets

| S-EPMC6559667 | biostudies-literature
| S-EPMC3191411 | biostudies-literature
| S-EPMC5112938 | biostudies-literature
| S-EPMC105116 | biostudies-literature
| S-EPMC4795698 | biostudies-literature
| S-EPMC4045327 | biostudies-literature
| S-EPMC6349502 | biostudies-literature
| S-EPMC6164957 | biostudies-literature
| S-EPMC7027967 | biostudies-literature
| S-EPMC3778852 | biostudies-other