Establishment and Expression of Cytokines in a Theileria annulata-Infected Bovine B Cell Line.

ABSTRACT: This study aimed to establish a pure single-cell Theileria annulata-infected B cell line for the assessment of cytokine production in transformed and lipopolysaccharide (LPS)-stimulated cells. Several studies have aimed to identify cell surface markers in T. annulata-transformed cells; however, no information on cytokine production in these cells is available. To investigate the potential of the transformed cells to produce cytokines and their potential responses to antigen-stimulation, we purified mature B cells (CD21) from the whole blood of cattle experimentally infected with the T. annulata Kashi strain by magnetic separation. The purity and specificity of the established cell line was assessed by the identification of specific cell surface markers (CD21, IgM, and

SUBMITTER: Rashid M 

PROVIDER: S-EPMC6562936 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature