Immunoprofiling of Breast Cancer Antigens Using Antibodies Derived from Local Lymph Nodes.

ABSTRACT: Tumor antigens are responsible for initiating an immune response in cancer patients, and their identification may provide new biomarkers for cancer diagnosis and targets for immunotherapy. The general use of serum antibodies to identify tumor antigens has several drawbacks, including dilution, complex formation, and background reactivity. In this study, antibodies were generated from antibody-secreting cells (ASC) present in tumor-draining lymph nodes of 20 breast cancer patients (ASC-probes) and were used to screen breast cancer cell lines and protein microarrays. Half of the ASC-probes reacted strongly against extracts of the MCF-7 breast cancer cell line, but each with a distinct antigen recognition profile. Three of the positive ASC-probes reacted differentially with recombinant antige

SUBMITTER: Young AR 

PROVIDER: S-EPMC6562983 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature