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Simple high-resolution NMR spectroscopy as a tool in molecular biology.

ABSTRACT: NMR is one of the major techniques for investigating the structure, dynamics and interactions between biomolecules. However, non-experts often experience NMR experimentation and data analysis as intimidating. We discuss a simple yet powerful NMR technique, the so-called chemical shift perturbation (CSP) analysis, as a tool to elucidate macromolecular interactions in small- and medium-sized complexes, including protein-protein, protein-drug, and protein-DNA/RNA interactions. We discuss current software packages for NMR data analysis and present a new interactive graphical tool implemented in CcpNmr AnalysisAssign version-3, which can drastically reduce the time required for the CSP analysis. Lastly, we illustrate the usefulness of a protein three-dimensional structure for interpretation of the CSP data.

SUBMITTER: Mureddu L

PROVIDER: S-EPMC6563160 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature

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Simple high-resolution NMR spectroscopy as a tool in molecular biology.

Mureddu Luca L Vuister Geerten W GW

The FEBS journal 20190214 11

NMR is one of the major techniques for investigating the structure, dynamics and interactions between biomolecules. However, non-experts often experience NMR experimentation and data analysis as intimidating. We discuss a simple yet powerful NMR technique, the so-called chemical shift perturbation (CSP) analysis, as a tool to elucidate macromolecular interactions in small- and medium-sized complexes, including protein-protein, protein-drug, and protein-DNA/RNA interactions. We discuss current so ...[more]

PMID: 30706658

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