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Formate Oxidase (FOx) from Aspergillus oryzae: One Catalyst Enables Diverse H2 O2 -Dependent Biocatalytic Oxidation Reactions.

ABSTRACT: An increasing number of biocatalytic oxidation reactions rely on H2 O2 as a clean oxidant. The poor robustness of most enzymes towards H2 O2 , however, necessitates more efficient systems for in?situ H2 O2 generation. In analogy to the well-known formate dehydrogenase to promote NADH-dependent reactions, we here propose employing formate oxidase (FOx) to promote H2 O2 -dependent enzymatic oxidation reactions. Even under non-optimised conditions, high turnover numbers for coupled FOx/peroxygenase catalysis were achieved.

SUBMITTER: Tieves F

PROVIDER: S-EPMC6563469 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature

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Formate Oxidase (FOx) from Aspergillus oryzae: One Catalyst Enables Diverse H2 O2 -Dependent Biocatalytic Oxidation Reactions.

Tieves Florian F Willot Sébastien Jean-Paul SJ van Schie Morten Martinus Cornelis Harald MMCH Rauch Marine Charlène Renée MCR Younes Sabry Hamdy Hamed SHH Zhang Wuyuan W Dong JiaJia J Gomez de Santos Patricia P Robbins John Mick JM Bommarius Bettina B Alcalde Miguel M Bommarius Andreas Sebastian AS Hollmann Frank F

Angewandte Chemie (International ed. in English) 20190430 23

An increasing number of biocatalytic oxidation reactions rely on H2 O2 as a clean oxidant. The poor robustness of most enzymes towards H2 O2 , however, necessitates more efficient systems for in situ H2 O2 generation. In analogy to the well-known formate dehydrogenase to promote NADH-dependent reactions, we here propose employing formate oxidase (FOx) to promote H2 O2 -dependent enzymatic oxidation reactions. ...[more]

PMID: 30945422

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