Perfluorocarbon-Based ¹⁹ F?MRI Nanoprobes for In?Vivo Multicolor Imaging.

ABSTRACT: In?vivo multicolor imaging is important for monitoring multiple biomolecular or cellular processes in biology. ¹⁹ F magnetic resonance imaging (MRI) is an emerging in?vivo imaging technique because it can non-invasively visualize ¹⁹ F nuclei without endogenous background signals. Therefore, ¹⁹ F?MRI probes capable of multicolor imaging are in high demand. Herein, we report five types of perfluorocarbon-encapsulated silica nanoparticles that show ¹⁹ F?NMR peaks with different chemical shifts. Three of the nanoprobes, which show spectrally distinct ¹⁹ F?NMR peaks with sufficient sensitivity, were selected for in?vivo multicolor ¹⁹ F?MRI. The nanoprobes exhibited ¹⁹ F?MRI signals with three colors in a living mouse. Our in?vivo multicolor system could be utilized for evaluating the effect of surface functional groups on the hepatic uptake in a mouse. This novel multicolor imaging technology will be a practical tool for elucidating in?vivo biomolecular networks by ¹⁹ F?MRI.

SUBMITTER: Akazawa K 

PROVIDER: S-EPMC6563715 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature