Regulatory effect of miR-144-3p on the function of human trabecular meshwork cells and fibronectin-1.

ABSTRACT: Role of microRNA (miR)-144-3p in regulating the function of human trabecular meshwork cells (HTMCs) and fibronectin-1 (FN-1) was investigated. HTM cell lines were divided into five groups, of which four groups established oxidative stress HTMC models and one served as a control group. The four model groups were transfected with miR-144-3p independent sequence, inhibitory sequence, over-expression sequence, and the blank group received no transfection. In addition, 40 primary open angle glaucoma patients treated in Xuzhou No. 1 People's Hospital were included in the observation group, and 40 healthy individuals were enrolled as a normal group. RT-qPCR was used for the detection of miR-144-3p expression in serum and cells of patients and healthy people in each group, western blot analysis for FN-1 expression in cells, CCK-8 kit for cell proliferation, and Transwell for cell invasion. The expression of serum miR-144-3p in the observation group was significantly lower than that in the normal group (P<0.05). The cell optical density value in the over-expression group was significantly higher than that in the other groups (P<0.05), and in the inhibition group was significantly lower than that in the other groups (P<0.05). The number of cell-penetration in the over-expression group was significantly higher than that in the other groups (P<0.05). The expression of FN-1 protein in the over-expression group was significantly lower than that in the other four groups (P<0.05), and the expression in the inhibition group was significantly higher than that in the other four groups (P<0.05). The over-expression of miR-144-3p promotes proliferation and invasion of HTMCs by inhibiting the expression of FN-1 in inoxidative stress HTMCs, and is a potential target for glaucoma treatment.

SUBMITTER: Yin R 

PROVIDER: S-EPMC6566054 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature