Dataset Information

Antibacterial Application on Staphylococcus aureus Using Antibiotic Agent/Zinc Oxide Nanorod Arrays/Polyethylethylketone Composite Samples.

ABSTRACT: In this study, zinc oxide (ZnO) nanorod arrays as antibiotic agent carriers were grown on polyetheretherketone (PEEK) substrates using a chemical synthesis method. With the concentration of ammonium hydroxide in the precursor solution kept at 4 M, ZnO nanorod arrays with diameters in the range of 100-400 nm and a loading density of 1.7 mg/cm² were grown onto the PEEK substrates. Their drug release profiles and the antibacterial properties of the antibiotic agent/ZnO/PEEK samples in the buffer solution were investigated. The results showed that the concentrations of antibiotic agents (ampicillin or vancomycin) released from the samples into the buffer solution were higher than the value of minimum inhibitory concentration of 90% for Staphylococcus aureus within the 96 h test. The bioactivities of ampicillin and vancomycin on substrates also showed around 40% and 80% on the Staphylococcus aureus, respectively. In the antibacterial activity test, sample with the suitable loading amount of antibiotic agent had a good inhibitory effect on the growth of Staphylococcus aureus.

SUBMITTER: Chen DW

PROVIDER: S-EPMC6566776 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature

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Publications

Antibacterial Application on <i>Staphylococcus aureus</i> Using Antibiotic Agent/Zinc Oxide Nanorod Arrays/Polyethylethylketone Composite Samples.

Chen Dave W DW Lee Kuan-Yi KY Tsai Min-Hua MH Lin Tung-Yi TY Chen Chien-Hao CH Cheng Kong-Wei KW

Nanomaterials (Basel, Switzerland) 20190508 5

In this study, zinc oxide (ZnO) nanorod arrays as antibiotic agent carriers were grown on polyetheretherketone (PEEK) substrates using a chemical synthesis method. With the concentration of ammonium hydroxide in the precursor solution kept at 4 M, ZnO nanorod arrays with diameters in the range of 100-400 nm and a loading density of 1.7 mg/cm<sup>2</sup> were grown onto the PEEK substrates. Their drug release profiles and the antibacterial properties of the antibiotic agent/ZnO/PEEK samples in th ...[more]

PMID: 31071920

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Project description:Is there a universal genetically programmed defense providing tolerance to antibiotics when bacteria grow as biofilms? A comparison between biofilms of three different bacterial species by transcriptomic and metabolomic approaches uncovered no evidence of one. Single-species biofilms of three bacterial species (Pseudomonas aeruginosa, Staphylococcus aureus, and Acinetobacter baumannii) were grown in vitro for three days then challenged with respective antibiotics (ciprofloxacin, daptomycin, tigecycline) for an additional 24 h. All three microorganisms displayed reduced susceptibility in biofilms compared to planktonic cultures. Global transcriptomic profiling of gene expression comparing biofilm to planktonic and antibiotic-treated biofilm to untreated biofilm was performed. Extracellular metabolites including 18 amino acids, glucose, lactate, acetate, formate, and ethanol were measured to characterize the utilization of carbon sources between biofilms, treated biofilms, and planktonic cells. While all three bacteria exhibited a species-specific signature of stationary phase, no conserved gene, gene set, or common functional pathway could be identified that changed consistently across the three microorganisms. Across the three species, glucose consumption was increased in biofilms compared to planktonic cells and alanine and aspartic acid utilization were decreased in biofilms compared to planktonic cells. The reasons for these changes were not readily apparent in the transcriptomes. No common shift in the utilization pattern of carbon sources was discerned when comparing untreated to antibiotic-exposed biofilms. Overall, our measurements do not support the existence of a common genetic or biochemical basis for biofilm tolerance against antibiotics. Rather, there are likely myriad genes, proteins, and metabolic pathways that influence the physiological state of microorganisms in biofilms contributing to antibiotic tolerance. The Staphylococcus aureus microarray data from the study described above is deposited here.

Dataset Information

Antibacterial Application on Staphylococcus aureus Using Antibiotic Agent/Zinc Oxide Nanorod Arrays/Polyethylethylketone Composite Samples.

Publications

Antibacterial Application on <i>Staphylococcus aureus</i> Using Antibiotic Agent/Zinc Oxide Nanorod Arrays/Polyethylethylketone Composite Samples.

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