Cloning and characterization of special HMW glutenin subunit genes from Aegilops longissima L. and their potential for wheat quality improvement.

ABSTRACT: Identification and cloning of new glutenin genes from wheat-related species can provide candidate gene resources for quality improvement of wheat. In this study, ten special high-molecular-weight glutenin subunits (HMW-GS), including five x-type (1S^l2x, 1S^l16x, 1S^l17x, 1S^l23x, and 1S^l25x) and five y-type (1S^l2y, 1S^l6y₁, 1S^l16y, 1S^l17y, and 1S^l23y) from Aegilops longissima L. (S^lS^l, 2n = 2x = 14) were identified, and their complete encoding genes were cloned by allelic-specific polymerase chain reaction (AS-PCR). The deduced amino acid (aa) residues of the x-type subunit genes ranged from 821 aa (2469 bp) to 941 aa (2829 bp), while those of th

SUBMITTER: Hu J 

PROVIDER: S-EPMC6570732 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature