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NCP activates chloroplast transcription by controlling phytochrome-dependent dual nuclear and plastidial switches.

ABSTRACT: Phytochromes initiate chloroplast biogenesis by activating genes encoding the photosynthetic apparatus, including photosynthesis-associated plastid-encoded genes (PhAPGs). PhAPGs are transcribed by a bacterial-type RNA polymerase (PEP), but how phytochromes in the nucleus activate chloroplast gene expression remains enigmatic. We report here a forward genetic screen in Arabidopsis that identified NUCLEAR CONTROL OF PEP ACTIVITY (NCP) as a necessary component of phytochrome signaling for PhAPG activation. NCP is dual-targeted to plastids and the nucleus. While nuclear NCP mediates the degradation of two repressors of chloroplast biogenesis, PIF1 and PIF3, NCP in plastids promotes the assembly of the PEP complex for PhAPG transcription. NCP and its paralog RCB are non-catalytic thioredoxin-like proteins that diverged in seed plants to adopt nonredundant functions in phytochrome signaling. These results support a model in which phytochromes control PhAPG expression through light-dependent double nuclear and plastidial switches that are linked by evolutionarily conserved and dual-localized regulatory proteins.

SUBMITTER: Yang EJ

PROVIDER: S-EPMC6570768 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature

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NCP activates chloroplast transcription by controlling phytochrome-dependent dual nuclear and plastidial switches.

Yang Emily J EJ Yoo Chan Yul CY Liu Jiangxin J Wang He H Cao Jun J Li Fay-Wei FW Pryer Kathleen M KM Sun Tai-Ping TP Weigel Detlef D Zhou Pei P Chen Meng M

Nature communications 20190614 1

Phytochromes initiate chloroplast biogenesis by activating genes encoding the photosynthetic apparatus, including photosynthesis-associated plastid-encoded genes (PhAPGs). PhAPGs are transcribed by a bacterial-type RNA polymerase (PEP), but how phytochromes in the nucleus activate chloroplast gene expression remains enigmatic. We report here a forward genetic screen in Arabidopsis that identified NUCLEAR CONTROL OF PEP ACTIVITY (NCP) as a necessary component of phytochrome signaling for PhAPG ac ...[more]

PMID: 31201314

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Dataset Information

NCP activates chloroplast transcription by controlling phytochrome-dependent dual nuclear and plastidial switches.

Publications

NCP activates chloroplast transcription by controlling phytochrome-dependent dual nuclear and plastidial switches.

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