Unknown

Dataset Information

0

Structure of the Prx6-subfamily 1-Cys peroxiredoxin from Sulfolobus islandicus.


ABSTRACT: Aerobic thermoacidophilic archaea belonging to the genus Sulfolobus harbor peroxiredoxins, thiol-dependent peroxidases that assist in protecting the cells from oxidative damage. Here, the crystal structure of the 1-Cys peroxiredoxin from Sulfolobus islandicus, named 1-Cys SiPrx, is presented. A 2.75?Å resolution data set was collected from a crystal belonging to space group P212121, with unit-cell parameters a = 86.8, b = 159.1, c = 189.3?Å, ? = ? = ? = 90°. The structure was solved by molecular replacement using the homologous Aeropyrum pernix peroxiredoxin (ApPrx) structure as a search model. In the crystal structure, 1-Cys SiPrx assembles into a ring-shaped decamer composed of five homodimers. This quaternary structure corresponds to the oligomeric state of the protein in solution, as observed by size-exclusion chromatography. 1-Cys SiPrx harbors only a single cysteine, which is the peroxidatic cysteine, and lacks both of the cysteines that are highly conserved in the C-terminal arm domain in other archaeal Prx6-subfamily proteins such as ApPrx and that are involved in the association of dimers into higher-molecular-weight decamers and dodecamers. It is thus concluded that the Sulfolobus Prx6-subfamily protein undergoes decamerization independently of arm-domain cysteines.

SUBMITTER: Stroobants S 

PROVIDER: S-EPMC6572100 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature

altmetric image

Publications

Structure of the Prx6-subfamily 1-Cys peroxiredoxin from Sulfolobus islandicus.

Stroobants Sander S   Van Molle Inge I   Saidi Queen Q   Jonckheere Karl K   Maes Dominique D   Peeters Eveline E  

Acta crystallographica. Section F, Structural biology communications 20190513 Pt 6


Aerobic thermoacidophilic archaea belonging to the genus Sulfolobus harbor peroxiredoxins, thiol-dependent peroxidases that assist in protecting the cells from oxidative damage. Here, the crystal structure of the 1-Cys peroxiredoxin from Sulfolobus islandicus, named 1-Cys SiPrx, is presented. A 2.75 Å resolution data set was collected from a crystal belonging to space group P2<sub>1</sub>2<sub>1</sub>2<sub>1</sub>, with unit-cell parameters a = 86.8, b = 159.1, c = 189.3 Å, α = β = γ = 90°. The  ...[more]

Similar Datasets

| S-EPMC5454432 | biostudies-literature
| S-EPMC6712394 | biostudies-literature
| S-EPMC2689034 | biostudies-literature
| S-EPMC4453537 | biostudies-literature
| S-EPMC3157427 | biostudies-literature
| S-EPMC6249222 | biostudies-literature
| S-EPMC2946923 | biostudies-literature
| S-EPMC1855697 | biostudies-literature
| S-EPMC6452263 | biostudies-literature
| S-EPMC7498313 | biostudies-literature