Ontology highlight
ABSTRACT:
SUBMITTER: Bai J
PROVIDER: S-EPMC6575577 | biostudies-literature | 2019 Jun
REPOSITORIES: biostudies-literature
Bai Jin J Wu Kenneth K Cao Meng-Han MH Yang Yingying Y Pan Yu Y Liu Hui H He Yizhou Y Itahana Yoko Y Huang Lan L Zheng Jun-Nian JN Pan Zhen-Qiang ZQ
Proceedings of the National Academy of Sciences of the United States of America 20190528 24
Human homolog of mouse double minute 2 (HDM2) is an oncogene frequently overexpressed in cancers with poor prognosis, but mechanisms of controlling its abundance remain elusive. In an unbiased biochemical search, we discovered <u>S</u>kp1-<u>C</u>ullin 1-<u>F</u>BXO22-ROC1 (SCF<sup>FBXO22</sup>) as the most dominating HDM2 E3 ubiquitin ligase from human proteome. The results of protein decay rate analysis, ubiquitination, siRNA-mediated silencing, and coimmunoprecipitation experiments support a ...[more]