Unknown

Dataset Information

0

Cisgenic overexpression of cytosolic glutamine synthetase improves nitrogen utilization efficiency in barley and prevents grain protein decline under elevated CO2.


ABSTRACT: Cytosolic glutamine synthetase (GS1) plays a central role in nitrogen (N) metabolism. The importance of GS1 in N remobilization during reproductive growth has been reported in cereal species but attempts to improve N utilization efficiency (NUE) by overexpressing GS1 have yielded inconsistent results. Here, we demonstrate that transformation of barley (Hordeum vulgare L.) plants using a cisgenic strategy to express an extra copy of native HvGS1-1 lead to increased HvGS1.1 expression and GS1 enzyme activity. GS1 overexpressing lines exhibited higher grain yields and NUE than wild-type plants when grown under three different N supplies and two levels of atmospheric CO2 . In contrast with the wild-type, the grain protein concentration in the GS1 overexpressing lines did not decline when plants were exposed to elevated (800-900 ?L/L) atmospheric CO2 . We conclude that an increase in GS1 activity obtained through cisgenic overexpression of HvGS1-1 can improve grain yield and NUE in barley. The extra capacity for N assimilation obtained by GS1 overexpression may also provide a means to prevent declining grain protein levels under elevated atmospheric CO2 .

SUBMITTER: Gao Y 

PROVIDER: S-EPMC6576097 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature

altmetric image

Publications

Cisgenic overexpression of cytosolic glutamine synthetase improves nitrogen utilization efficiency in barley and prevents grain protein decline under elevated CO<sub>2</sub>.

Gao Yajie Y   de Bang Thomas C TC   Schjoerring Jan K JK  

Plant biotechnology journal 20181227 7


Cytosolic glutamine synthetase (GS1) plays a central role in nitrogen (N) metabolism. The importance of GS1 in N remobilization during reproductive growth has been reported in cereal species but attempts to improve N utilization efficiency (NUE) by overexpressing GS1 have yielded inconsistent results. Here, we demonstrate that transformation of barley (Hordeum vulgare L.) plants using a cisgenic strategy to express an extra copy of native HvGS1-1 lead to increased HvGS1.1 expression and GS1 enzy  ...[more]

Similar Datasets

2019-04-01 | E-MTAB-6303 | biostudies-arrayexpress
| S-EPMC4378633 | biostudies-literature
| S-EPMC3376133 | biostudies-literature
2010-03-01 | GSE15122 | GEO
2014-11-28 | GSE62124 | GEO
| S-EPMC5441914 | biostudies-literature
| S-EPMC4942471 | biostudies-literature
| S-EPMC9303220 | biostudies-literature
| S-EPMC8448750 | biostudies-literature
| S-EPMC4265158 | biostudies-literature