Tandem Affinity Purification and Mass Spectrometry (TAP-MS) for the Analysis of Protein Complexes.
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ABSTRACT:
SUBMITTER: Adelmant G
PROVIDER: S-EPMC6579647 | biostudies-literature | 2019 Jun
REPOSITORIES: biostudies-literature
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Tandem Affinity Purification and Mass Spectrometry (TAP-MS) for the Analysis of Protein Complexes.
Adelmant Guillaume G Garg Brijesh K BK Tavares Maria M Card Joseph D JD Marto Jarrod A JA
Current protocols in protein science 20190201 1
Affinity purification followed by mass spectrometry has become the technique of choice to identify binding partners in biochemical complexes isolated from a physiologic cellular context. In this report we detail our protocol for tandem affinity purification (TAP) primarily based on the use of the FLAG and HA peptide epitopes, with a particular emphasis on factors affecting yield and specificity, as well as steps to implement an automated version of the TAP procedure. © 2019 by John Wiley & Sons, ...[more]
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