Dataset Information

De novo transcriptome analysis of gene responses to pest feeding in leaves of Panax ginseng C. A. Meyer.

ABSTRACT: The aim of the present study was to investigate the transcriptomic differences between Panax ginseng [Renshen (RS)] plants bitten by pests (n=3, test group; samples defined as RS11?13) or not (n=3, control group; samples defined as RS1?3) using de novo RNA sequencing on an Illumina HiSeq™ 2000 platform. A total of 51,097,386 (99.6%), 49,310,564 (99.5%), 59,192,372 (99.6%), 60,338,540 (99.5%), 56,976,410 (99.6%) and 54,226,588 (99.6%) clean reads were obtained for RS11, RS12, RS13, RS1, RS2 and RS3, respectively. De novo assembly generated 370,267 unigenes, 927 of which were differentially expressed genes (DEGs), including 782 significantly upregulated and 145 significantly downregulated genes. Function enrichment analysis revealed that these DEGs were located in 28 significantly enriched Kyoto Encyclopedia of Genes and Genomes pathways, including phenylpropanoid biosynthesis (for example, TRINITY_DN30766_c0_g2_i1, encoding peroxidase 20) and mitogen?activated protein kinase (MAPK) signaling (TRINITY_DN85589_c0_g1_i1, encoding WRKY transcription factor 75). Weighted gene co?expression network analysis identified modules including TRINITY_DN85589_c0_g1_i1, TRINITY_DN58279_c0_g1_i1 [encoding aspartyl protease (AP)] and TRINITY_DN74866_c0_g2_i1 [encoding 12?oxophytodienoate reductase (OPR)] that may be the most significantly associated with pest responses. In this module, TRINITY_DN85589_c0_g1_i1 may co?express with TRINITY_DN58279_c0_g1_i1 or TRINITY_DN74866_c0_g2_i1. WRYK and AP have been suggested to promote the activity of antioxidant peroxidase. Collectively, the findings from the present study suggested that a MAPK?WRKY?OPR/AP?peroxidase signaling pathway may be a potentially important mechanism underlying defense responses against pests in ginseng plants.

SUBMITTER: Xi G

PROVIDER: S-EPMC6580019 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature

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De novo transcriptome analysis of gene responses to pest feeding in leaves of Panax ginseng C. A. Meyer.

Xi Guangsheng G Wang Yanling Y Yin Le L Wang Yunjia Y Zhou Shengxue S

Molecular medicine reports 20190522 1

The aim of the present study was to investigate the transcriptomic differences between Panax ginseng [Renshen (RS)] plants bitten by pests (n=3, test group; samples defined as RS11‑13) or not (n=3, control group; samples defined as RS1‑3) using de novo RNA sequencing on an Illumina HiSeq™ 2000 platform. A total of 51,097,386 (99.6%), 49,310,564 (99.5%), 59,192,372 (99.6%), 60,338,540 (99.5%), 56,976,410 (99.6%) and 54,226,588 (99.6%) clean reads were obtained for RS11, RS12, RS13, RS1, RS2 and R ...[more]

PMID: 31180519

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Project description:We aimed to investigate the effects of genome, age, and soil factors on cultivated Panax ginseng C. A. Meyer (CPG) compounds under identical climate and agronomic practices. Eight populations of CPG from different years and rhizosphere soils were collected from garden and cropland in the city of Ji'an, China. Inter-simple sequence repeat (ISSR) primers were used to detect genetic diversity and identity, and soil microbial community diversity. Soil enzyme activities and nutrients were also measured. The contents of total ginsenosides (TG), Rg1, Re, Rf, Rd, and ginsenoside extractions of CPG were analyzed by spectrophotometry and HPLC. The relative importance of each factor was analyzed by mathematical methods such as correlation analysis, stepwise line regression, and path analysis. Regression equations of similarity values of HPLC fingerprint (SVHF), richness index of HPLC fingerprint (RIHF) and the TG, Rg1, Re, Rf, and Rd contents with their respective significant correlation factors were obtained. For SVHF, the relative importance is age>microbial community diversity>genetic diversity. For RIHF, the relative importance is age>genetic diversity>microbial community diversity. For TG, Rg1, and Rf contents, the relative importance is age>microbial community diversity. Ginseng age and genetic identity influenced Rd content, and age was more important. Total phosphorus was the only directly negative effect on Re. According to regression equations and path analysis, increasing age and decreasing Shannon (H') could improve the TG, Rg1, and Rf contents, with little effect on SVHF. Adding age, genetic diversity, and decreasing Shannon (H') increased RIHF. Adding age and genetic identity could also improve Rd content. Appropriate decreases in total phosphorus might increase Re content. These findings are significant for CPG scientific cultivation methods, through which CPG bioactive ingredients could be finely controlled via regulation of genotypes and cultural conditions.

Project description:BACKGROUND: Panax ginseng C. A. Meyer is one of the most widely used medicinal plants. Complete genome information for this species remains unavailable due to its large genome size. At present, analysis of expressed sequence tags is still the most powerful tool for large-scale gene discovery. The global expressed sequence tags from P. ginseng tissues, especially those isolated from stems, leaves and flowers, are still limited, hindering in-depth study of P. ginseng. RESULTS: Two 454 pyrosequencing runs generated a total of 2,423,076 reads from P. ginseng roots, stems, leaves and flowers. The high-quality reads from each of the tissues were independently assembled into separate and shared contigs. In the separately assembled database, 45,849, 6,172, 4,041 and 3,273 unigenes were only found in the roots, stems, leaves and flowers database, respectively. In the jointly assembled database, 178,145 unigenes were observed, including 86,609 contigs and 91,536 singletons. Among the 178,145 unigenes, 105,522 were identified for the first time, of which 65.6% were identified in the stem, leaf or flower cDNA libraries of P. ginseng. After annotation, we discovered 223 unigenes involved in ginsenoside backbone biosynthesis. Additionally, a total of 326 potential cytochrome P450 and 129 potential UDP-glycosyltransferase sequences were predicted based on the annotation results, some of which may encode enzymes responsible for ginsenoside backbone modification. A BLAST search of the obtained high-quality reads identified 14 potential microRNAs in P. ginseng, which were estimated to target 100 protein-coding genes, including transcription factors, transporters and DNA binding proteins, among others. In addition, a total of 13,044 simple sequence repeats were identified from the 178,145 unigenes. CONCLUSIONS: This study provides global expressed sequence tags for P. ginseng, which will contribute significantly to further genome-wide research and analyses in this species. The novel unigenes identified here enlarge the available P. ginseng gene pool and will facilitate gene discovery. In addition, the identification of microRNAs and the prediction of targets from this study will provide information on gene transcriptional regulation in P. ginseng. Finally, the analysis of simple sequence repeats will provide genetic makers for molecular breeding and genetic applications in this species.

Dataset Information

De novo transcriptome analysis of gene responses to pest feeding in leaves of Panax ginseng C. A. Meyer.

Publications

De novo transcriptome analysis of gene responses to pest feeding in leaves of Panax ginseng C. A. Meyer.

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