Ontology highlight
ABSTRACT:
SUBMITTER: Manolopoulou M
PROVIDER: S-EPMC6580245 | biostudies-literature | 2019 May
REPOSITORIES: biostudies-literature
Manolopoulou Marika M Matlock Brittany K BK Nlandu-Khodo Stellor S Simmons Alan J AJ Lau Ken S KS Phillips-Mignemi Melanie M Ivanova Alla A Alford Catherine E CE Flaherty David K DK Gewin Leslie S LS
American journal of physiology. Renal physiology 20190213 5
Flow cytometry studies on injured kidney tubules are complicated by the low yield of nucleated single cells. Furthermore, cell-specific responses such as cell cycle dynamics in vivo have conventionally relied on indirect immunohistochemistry and proximal tubule markers that may be downregulated in injury. Here, we report a new tissue dissociation protocol for the kidney with an early fixation step that greatly enhances the yield of single cells. Genetic labeling of the proximal tubule with eithe ...[more]