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Melibiosamine, a novel oligosaccharide, suppresses mitogen-induced IL-2 production via inactivation of NFAT and NF?B in Jurkat cells.

ABSTRACT: d-Glucosamine (GlcNH₂) and several of its derivatives are known to possess immunosuppressive activities in various immune cell lines. The novel GlcNH₂-containing oligosaccharide Gal?1-6GlcNH₂ (designated melibiosamine; MelNH₂) is expected to be immunosuppressive also. In Jurkat cells (immortalized human T lymphocytes), interleukin 2 (IL-2) production (an index of the T-cell immune response) can be induced by stimulation with a mitogen, such as concanavalin A. Here, we compared the effects of GlcNH₂ and MelNH₂ on concanavalin A-induced IL-2 production (CIIP) in Jurkat cells and found that GlcNH₂ and MelNH₂ at millimolar levels both significantly suppressed CIIP without affecting cell viability. When we examined the effects of GlcNH₂ and MelNH₂ on the activation of the three transcription factors required for CIIP-NFAT (nuclear factor of activated T-cells), NF?B (nuclear factor kappa-light-chain-enhancer of activated B cells), and AP-1 (activator protein 1)-we found that GlcNH₂ and MelNH₂ both suppressed CIIP by inhibiting the activation of NFAT and NF?B, but, unlike GlcNH₂, MelNH₂ also promoted the activation of AP-1. These results suggest that MelNH₂ may be a potentially useful lead compound for development as an immunosuppressive or anti-inflammatory drug.

SUBMITTER: Fujita K

PROVIDER: S-EPMC6580327 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature

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Melibiosamine, a novel oligosaccharide, suppresses mitogen-induced IL-2 production via inactivation of NFAT and NFκB in Jurkat cells.

Fujita Kazuki K Tanaka Susumu S Iizumi Kyoichi K Akiyama Shuri S Uchida Kaoru K Ogata Makoto M Aoki Daichi D Hosomi Osamu O Kubohara Yuzuru Y

Biochemistry and biophysics reports 20190614

d-Glucosamine (GlcNH2) and several of its derivatives are known to possess immunosuppressive activities in various immune cell lines. The novel GlcNH2-containing oligosaccharide Galα1-6GlcNH2 (designated melibiosamine; MelNH2) is expected to be immunosuppressive also. In Jurkat cells (immortalized human T lymphocytes), interleukin 2 (IL-2) production (an index of the T-cell immune response) can be induced by stimulation with a mitogen, such as concanav ...[more]

PMID: 31431927

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Dataset Information

Melibiosamine, a novel oligosaccharide, suppresses mitogen-induced IL-2 production via inactivation of NFAT and NF?B in Jurkat cells.

Publications

Melibiosamine, a novel oligosaccharide, suppresses mitogen-induced IL-2 production via inactivation of NFAT and NFκB in Jurkat cells.

Similar Datasets

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