Dataset Information

New microsatellites for the Atacama Desert endemic Balsamocarpon brevifolium (Fabaceae).

ABSTRACT: Premise:Algarrobilla (Balsamocarpon brevifolium, Fabaceae) is an endemic xerophytic shrub restricted to the Atacama Desert in northern Chile. Extensive utilization of the region for coal production has endangered this species. Conservation efforts are underway, with genetic diversity analyses being key to the restoration of these populations. Methods and Results:Fifteen new microsatellite markers were developed for B. brevifolium and used to analyze three populations from the Atacama and Coquimbo regions in Chile. Microsatellites were highly polymorphic, with an average of 5.77 alleles per marker and an average level of expected heterozygosity of 0.72. These markers were evaluated and cross-amplified on two related species (Senna cumingii and Caesalpinia angulata) with partial success. Conclusions:The development of this set of markers permits an extensive study of B. brevifolium populations for conservation purposes.

SUBMITTER: Ravest G

PROVIDER: S-EPMC6580986 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature

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Publications

New microsatellites for the Atacama Desert endemic <i>Balsamocarpon brevifolium</i> (Fabaceae).

Ravest Gonzalo G León-Lobos Pedro P Aguirre Carlos C Hernández José J Bolados Gustavo G Castro María Herminia MH Silva Sergio S Hinrichsen Patricio P

Applications in plant sciences 20190611 6

<h4>Premise</h4>Algarrobilla (<i>Balsamocarpon brevifolium</i>, Fabaceae) is an endemic xerophytic shrub restricted to the Atacama Desert in northern Chile. Extensive utilization of the region for coal production has endangered this species. Conservation efforts are underway, with genetic diversity analyses being key to the restoration of these populations.<h4>Methods and results</h4>Fifteen new microsatellite markers were developed for <i>B. brevifolium</i> and used to analyze three populations ...[more]

PMID: 31236318

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Project description:In a desert, plants as holobionts quickly respond to resource pulses like precipitation. However, little is known on how environment and plants modulate the rhizosphere-associated microbiome. As a model species to represent the Atacama Desert bloom, Cistanthe longiscapa (Montiaceae family) was selected to study the influence of abiotic and biotic environment on the diversity and structure of the microbiota associated to its rhizosphere. We analyzed the rhizosphere and soil microbiome along a North-South precipitation gradient and between a dry and rainy year by using Illumina high-throughput sequencing of 16S rRNA gene fragments and ITS2 regions for prokaryotes and fungi, respectively. In the rhizosphere of C. longiscapa the microbiota clearly differs in composition and structure from the surrounding bulk soil. The fungal and bacterial communities respond differently to environmental conditions. The diversity and richness of fungal OTUs were negatively correlated with aridity, as predicted. The community structure was predominantly influenced by other soil characteristics (pH, organic matter content) but not by aridity. In contrast, diversity, composition, and structure of the bacterial community were not influenced by aridity or any other evaluated soil parameter. These findings coincide with the identification of mainly site-specific microbial communities, not shared along the sites. These local communities contain a group of OTUs, which are exclusive to the rhizosphere of each site and presumably vertically inherited as seed endophytes. Their ecological functions and dispersal mechanisms remain unclear. The analysis of co-occurrence patterns highlights the strong effect of the desert habitat over the soil- and rhizosphere-microbiome. The site-independent enrichment of only a small bacterial cluster consistently associated with the rhizosphere of C. longiscapa further supports this conclusion. In a rainy year, the rhizosphere microbiota significantly differed from bulk and bare soil, whereas in a dry year, the community structure of the former rhizosphere approximates to the one found in the bulk. In the context of plant-microbe interactions in desert environments, our study contributes new insights into the importance of aridity in microbial community structure and composition, discovering the influence of other soil parameters in this complex dynamic network, which needs further to be investigated.

Dataset Information

New microsatellites for the Atacama Desert endemic Balsamocarpon brevifolium (Fabaceae).

Publications

New microsatellites for the Atacama Desert endemic <i>Balsamocarpon brevifolium</i> (Fabaceae).

Similar Datasets

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