Ontology highlight
ABSTRACT:
SUBMITTER: Migawa MT
PROVIDER: S-EPMC6582325 | biostudies-literature | 2019 Jun
REPOSITORIES: biostudies-literature
Migawa Michael T MT Shen Wen W Wan W Brad WB Vasquez Guillermo G Oestergaard Michael E ME Low Audrey A De Hoyos Cheryl L CL Gupta Ruchi R Murray Susan S Tanowitz Michael M Bell Melanie M Nichols Joshua G JG Gaus Hans H Liang Xue-Hai XH Swayze Eric E EE Crooke Stanley T ST Seth Punit P PP
Nucleic acids research 20190601 11
Phosphorothioate-modified antisense oligonucleotides (PS-ASOs) interact with a host of plasma, cell-surface and intracellular proteins which govern their therapeutic properties. Given the importance of PS backbone for interaction with proteins, we systematically replaced anionic PS-linkages in toxic ASOs with charge-neutral alkylphosphonate linkages. Site-specific incorporation of alkyl phosphonates altered the RNaseH1 cleavage patterns but overall rates of cleavage and activity versus the on-ta ...[more]