The Ussing chamber system for measuring intestinal permeability in health and disease.

ABSTRACT:

Background

The relationship between intestinal epithelial integrity and the development of intestinal disease is of increasing interest. A reduction in mucosal integrity has been associated with ulcerative colitis, Crohn's disease and potentially could have links with colorectal cancer development. The Ussing chamber system can be utilised as a valuable tool for measuring gut integrity. Here we describe step-by-step methodology required to measure intestinal permeability of both mouse and human colonic tissue samples ex vivo, using the latest equipment and software. This system can be modified to accommodate other tissues.

Methods

An Ussing chamber was constructed and adapted to support both mouse and human tissue to measure intestinal permeability, using paracellular flux a

SUBMITTER: Thomson A 

PROVIDER: S-EPMC6585111 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature