S-EPMC6585615 - Evaluation of Effects of TGF-?1 Inhibition on Gastric Cancer in Nude Mice by Diffusion Kurtosis Imaging and In-Line X-ray Phase Contrast Imaging With Sequential Histology. - OmicsDI

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Dataset Information

Evaluation of Effects of TGF-?1 Inhibition on Gastric Cancer in Nude Mice by Diffusion Kurtosis Imaging and In-Line X-ray Phase Contrast Imaging With Sequential Histology.

ABSTRACT:

Background

Accurate and complete response evaluation after treatment is important to implement individualized therapy for gastric cancer.

Purpose

To investigate the effectiveness of diffusion kurtosis imaging (DKI) and in-line X-ray phase contrast imaging (ILXPCI) in the assessment of the therapeutic efficacy by transforming growth factor beta 1 (TGF-?1) inhibition.

Study type

Prospective animal study.

Animal model

Thirty nude mice subcutaneous xenotransplantation tumor model of gastric cancer for DKI and 10 peritoneal metastasis nude mice model for ILXPCI.

Field strength/sequence

Examinations before and serially at 7, 14, 21, and 28 days after TGF-?1 inhibition treatment were performed at 3T MRI including T₂ -weighted imaging (T₂ WI) and DKI with five b values of 0, 500, 1000, 1500, 2000 s/mm² ; ILXPCI examinations were performed at 14 days after treatment.

Assessment

DKI parameters (apparent diffusion coefficient [ADC], diffusivity [D] and kurtosis [K]) were calculated by two experienced radiologists after postprocessing.

Statistical tests

For the differences in all the parameters between the baseline and each timepoint for both the treated and the control mice, the Mann-Whitney test was used. The Spearman correlation test was used to evaluate correlations among the DKI parameters and corresponding pathologic necrosis fraction (NF).

Results

ADC, D, and K values were significantly different between the two groups after treatment (P?Data conclusionDKI may be used to evaluate the complete course therapeutic effects of gastric cancer induced by TGF-?1 inhibition, and the ILXPCI technique will improve the tumor microstructure resolution.

Level of evidence

1 Technical Efficacy: Stage 4 J. Magn. Reson. Imaging 2019;49:1553-1564.

SUBMITTER: Shi B

PROVIDER: S-EPMC6585615 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature

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Publications

Evaluation of Effects of TGF-β1 Inhibition on Gastric Cancer in Nude Mice by Diffusion Kurtosis Imaging and In-Line X-ray Phase Contrast Imaging With Sequential Histology.

Shi Bowen B Yuan Fei F Yan Fuhua F Zhang Huan H Pan Zilai Z Chen Weibo W Wang Guilong G Tan Jingwen J Zhang Yang Y Ren Yuqi Y Du Lianjun L

Journal of magnetic resonance imaging : JMRI 20181006 6

<h4>Background</h4>Accurate and complete response evaluation after treatment is important to implement individualized therapy for gastric cancer.<h4>Purpose</h4>To investigate the effectiveness of diffusion kurtosis imaging (DKI) and in-line X-ray phase contrast imaging (ILXPCI) in the assessment of the therapeutic efficacy by transforming growth factor beta 1 (TGF-β1) inhibition.<h4>Study type</h4>Prospective animal study.<h4>Animal model</h4>Thirty nude mice subcutaneous xenotransplantation tu ...[more]

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