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Development and Validation of a Perfect KASP Marker for Fusarium Head Blight Resistance Gene Fhb1 in Wheat.


ABSTRACT: Fusarium head blight (FHB) is a devastating wheat disease with a significant economic impact. Fhb1 is the most important large effect and stable QTL for FHB resistance. A pore-forming toxin-like (PFT) gene was recently identified as an underlying gene for Fhb1 resistance. In this study, we developed and validated a PFT-based Kompetitive allele specific PCR (KASP) marker for Fhb1. The KASP marker, PFT_KASP, was used to screen 298 diverse wheat breeding lines and cultivars. The KASP clustering results were compared with gel-based gene specific markers and the widely used linked STS marker, UMN10. Eight disagreements were found between PFT_KASP and UMN10 assays among the tested lines. Based on the genotyping and sequencing of genes in the Fhb1 region, these genotypes were found to be common with a previously characterized susceptible haplotype. Therefore, our results indicate that PFT_KASP is a perfect diagnostic marker for Fhb1 and would be a valuable tool for introgression and pyramiding of FHB resistance in wheat cultivars.

SUBMITTER: Singh L 

PROVIDER: S-EPMC6586189 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature

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Development and Validation of a Perfect KASP Marker for Fusarium Head Blight Resistance Gene <i>Fhb1</i> in Wheat.

Singh Lovepreet L   Anderson James A JA   Chen Jianli J   Gill Bikram S BS   Tiwari Vijay K VK   Rawat Nidhi N  

The plant pathology journal 20190601 3


Fusarium head blight (FHB) is a devastating wheat disease with a significant economic impact. <i>Fhb1</i> is the most important large effect and stable QTL for FHB resistance. A pore-forming toxin-like (<i>PFT</i>) gene was recently identified as an underlying gene for <i>Fhb1</i> resistance. In this study, we developed and validated a <i>PFT</i>-based Kompetitive allele specific PCR (KASP) marker for <i>Fhb1</i>. The KASP marker, PFT_KASP, was used to screen 298 diverse wheat breeding lines and  ...[more]

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