HDL protects against myocardial ischemia reperfusion injury via miR-34b and miR-337 expression which requires STAT3.

ABSTRACT:

Purpose

High density lipoprotein (HDL) protects against myocardial infarction via mechanisms that remain unclear. STAT3 (signal transducer and activator of transcription 3) plays a key role in HDL-induced cardioprotection. In the heart, microRNAs (miRNAs) are involved in ischemia reperfusion injury. We therefore investigated whether the cardioprotective effect of HDL modulates miRNAs as a downstream target of STAT3 activation.

Methods

STAT3 cardiomyocyte deficient mice (STAT3-KO) and wildtype littermates (STAT3-WT) were submitted to left coronary ligature and reperfused (IR) with or without injection of HDL. Infarct size (IS) was determined and cardiac miRNA expression was evaluated after reperfusion in sham, IR and IR+HDL hearts by microarray analysis. In vitro, neonatal ra

SUBMITTER: Pedretti S 

PROVIDER: S-EPMC6586303 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature