Ontology highlight
ABSTRACT:
SUBMITTER: Taylor T
PROVIDER: S-EPMC6586679 | biostudies-literature | 2019 Jun
REPOSITORIES: biostudies-literature
Taylor Tracy T Lee Emma R ER Nykoluk Mikaela M Enns Eric E Liang Binhua B Capina Rupert R Gauthier Marie-Krystel MK Domselaar Gary Van GV Sandstrom Paul P Brooks James J Ji Hezhao H
Scientific reports 20190620 1
Conventional HIV drug resistance (HIVDR) genotyping utilizes Sanger sequencing (SS) methods, which are limited by low data throughput and the inability of detecting low abundant drug resistant variants (LADRVs). Here we present a next generation sequencing (NGS)-based HIVDR typing platform that leverages the advantages of Illumina MiSeq and HyDRA Web. The platform consists of a fully validated sample processing protocol and HyDRA web, an open web portal that allows automated customizable NGS-bas ...[more]