Ontology highlight
ABSTRACT:
SUBMITTER: Brunner E
PROVIDER: S-EPMC6587125 | biostudies-literature | 2019 Jun
REPOSITORIES: biostudies-literature
Brunner Erich E Yagi Ryohei R Debrunner Marc M Beck-Schneider Dezirae D Burger Alexa A Escher Eliane E Mosimann Christian C Hausmann George G Basler Konrad K
Life science alliance 20190613 3
CRISPR-Cas9-based genome editing has transformed the life sciences, enabling virtually unlimited genetic manipulation of genomes: The RNA-guided Cas9 endonuclease cuts DNA at a specific target sequence and the resulting double-strand breaks are mended by one of the intrinsic cellular repair pathways. Imprecise double-strand repair will introduce random mutations such as indels or point mutations, whereas precise editing will restore or specifically edit the locus as mandated by an endogenous or ...[more]