Ontology highlight
ABSTRACT: Background
In high-risk populations, allergen-specific prophylaxis could protect from sensitization and subsequent development of allergic disease. However, such treatment might itself induce sensitization and allergies, thus requiring hypoallergenic vaccine formulations. We here characterized the preventive potential of virus-like nanoparticles (VNP) expressing surface-exposed or shielded allergens.Methods
Full-length major mugwort pollen allergen Art v 1 was selectively targeted either to the surface or to the inner side of the lipid bilayer envelope of VNP. Upon biochemical and immunological analysis, their preventive potential was determined in a humanized mouse model of mugwort pollen allergy.Results
Virus-like nanoparticles expressing shielded version of Art v 1, in contrast to those expressing surface-exposed Art v 1, were hypoallergenic as they hardly induced degranulation of rat basophil leukemia cells sensitized with Art v 1-specific mouse or human IgE. Both VNP versions induced proliferation and cytokine production of allergen-specific T cells in vitro. Upon intranasal application in mice, VNP expressing surface-exposed but not shielded allergen induced allergen-specific antibodies, including IgE. Notably, preventive treatment with VNP expressing shielded allergen-protected mice from subsequent sensitization with mugwort pollen extract. Protection was associated with a Th1/Treg-dominated cytokine response, increased Foxp3+ Treg numbers in lungs, and reduced lung resistance when compared to mice treated with empty particles.Conclusion
Virus-like nanoparticles represent a novel and versatile platform for the in vivo delivery of allergens to selectively target T cells and prevent allergies without inducing allergic reactions or allergic sensitization.
SUBMITTER: Kratzer B
PROVIDER: S-EPMC6587790 | biostudies-literature | 2019 Feb
REPOSITORIES: biostudies-literature
Kratzer Bernhard B Köhler Cordula C Hofer Sandra S Smole Ursula U Trapin Doris D Iturri Jagoba J Pum Dietmar D Kienzl Philip P Elbe-Bürger Adelheid A Gattinger Pia P Mittermann Irene I Linhart Birgit B Gadermaier Gabriele G Jahn-Schmid Beatrice B Neunkirchner Alina A Valenta Rudolf R Pickl Winfried F WF
Allergy 20181105 2
<h4>Background</h4>In high-risk populations, allergen-specific prophylaxis could protect from sensitization and subsequent development of allergic disease. However, such treatment might itself induce sensitization and allergies, thus requiring hypoallergenic vaccine formulations. We here characterized the preventive potential of virus-like nanoparticles (VNP) expressing surface-exposed or shielded allergens.<h4>Methods</h4>Full-length major mugwort pollen allergen Art v 1 was selectively targete ...[more]