Dataset Information

Draft Genome Sequence of FK506-Producing Streptomyces tsukubensis Strain VKM Ac-2618D.

ABSTRACT: The 23-membered macrolide tacrolimus (FK506) is an important immunosuppressant that is widely used in the prevention of graft rejection and in the treatment of inflammatory skin diseases and immune diseases. We report here the draft genome sequence of the FK506 producer Streptomyces tsukubensis VKM Ac-2618D.

SUBMITTER: Poshekhontseva VY

PROVIDER: S-EPMC6588045 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature

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Draft Genome Sequence of FK506-Producing <i>Streptomyces tsukubensis</i> Strain VKM Ac-2618D.

Poshekhontseva Veronika Y VY Bragin Eugeny Y EY Fokina Victoria V VV Shtratnikova Victoriya Y VY Starodumova Irina P IP Tarlachkov Sergey V SV Donova Marina V MV

Microbiology resource announcements 20190613 24

The 23-membered macrolide tacrolimus (FK506) is an important immunosuppressant that is widely used in the prevention of graft rejection and in the treatment of inflammatory skin diseases and immune diseases. We report here the draft genome sequence of the FK506 producer <i>Streptomyces tsukubensis</i> VKM Ac-2618D. ...[more]

PMID: 31196928

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Project description:BackgroundAerobic side chain degradation of phytosterols by actinobacteria is the basis for the industrial production of androstane steroids which are the starting materials for the synthesis of steroid hormones. A native strain of Mycobacterium sp. VKM Ac-1817D effectively produces 9α-hydroxyandrost-4-ene-3,17-dione (9-OH-AD) from phytosterol, but also is capable of slow steroid core degradation. However, the set of the genes with products that are involved in phytosterol oxidation, their organisation and regulation remain poorly understood.ResultsHigh-throughput sequencing of the global transcriptomes of the Mycobacterium sp. VKM Ac-1817D cultures grown with or without phytosterol was carried out. In the presence of phytosterol, the expression of 260 genes including those related to steroid catabolism pathways significantly increased. Two of the five genes encoding the oxygenase unit of 3-ketosteroid-9α-hydroxylase (kshA) were highly up-regulated in response to phytosterol (55- and 25-fold, respectively) as well as one of the two genes encoding its reductase subunit (kshB) (40-fold). Only one of the five putative genes encoding 3-ketosteroid-∆1-dehydrogenase (KstD_1) was up-regulated in the presence of phytosterol (61-fold), but several substitutions in the conservative positions of its product were revealed. Among the genes over-expressed in the presence of phytosterol, several dozen genes did not possess binding sites for the known regulatory factors of steroid catabolism. In the promoter regions of these genes, a regularly occurring palindromic motif was revealed. The orthologue of TetR-family transcription regulator gene Rv0767c of M. tuberculosis was identified in Mycobacterium sp. VKM Ac-1817D as G155_05115.ConclusionsHigh expression levels of the genes related to the sterol side chain degradation and steroid 9α-hydroxylation in combination with possible defects in KstD_1 may contribute to effective 9α-hydroxyandrost-4-ene-3,17-dione accumulation from phytosterol provided by this biotechnologically relevant strain. The TetR-family transcription regulator gene G155_05115 presumably associated with the regulation of steroid catabolism. The results are of significance for the improvement of biocatalytic features of the microbial strains for the steroid industry.

Dataset Information

Draft Genome Sequence of FK506-Producing Streptomyces tsukubensis Strain VKM Ac-2618D.

Publications

Draft Genome Sequence of FK506-Producing <i>Streptomyces tsukubensis</i> Strain VKM Ac-2618D.

Similar Datasets

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