Ontology highlight
ABSTRACT:
SUBMITTER: Srinivasan M
PROVIDER: S-EPMC6588348 | biostudies-literature | 2019 Jun
REPOSITORIES: biostudies-literature
Srinivasan Madhusudhan M Petela Naomi J NJ Scheinost Johanna C JC Collier James J Voulgaris Menelaos M B Roig Maurici M Beckouët Frederic F Hu Bin B Nasmyth Kim A KA
eLife 20190621
Cohesin's association with chromosomes is determined by loading dependent on the Scc2/4 complex and release due to Wapl. We show here that Scc2 also actively maintains cohesin on chromosomes during G1 in <i>S. cerevisiae</i> cells. It does so by blocking a Wapl-independent release reaction that requires opening the cohesin ring at its Smc3/Scc1 interface as well as the D loop of Smc1's ATPase. The Wapl-independent release mechanism is switched off as cells activate Cdk1 and enter G2/M and cannot ...[more]