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DnaJB6 is a RanGTP-regulated protein required for microtubule organization during mitosis.


ABSTRACT: Bipolar spindle organization is essential for the faithful segregation of chromosomes during cell division. This organization relies on the collective activities of motor proteins. The minus-end-directed dynein motor complex generates spindle inward forces and plays a major role in spindle pole focusing. The dynactin complex regulates many dynein functions, increasing its processivity and force production. Here, we show that DnaJB6 is a novel RanGTP-regulated protein. It interacts with the dynactin subunit p150Glued (also known as DCTN1) in a RanGTP-dependent manner specifically in M-phase, and promotes spindle pole focusing and dynein force generation. Our data suggest a novel mechanism by which RanGTP regulates dynein activity during M-phase.

SUBMITTER: Rosas-Salvans M 

PROVIDER: S-EPMC6589090 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature

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