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Sensitive method for glycosaminoglycan analysis of tissue sections.


ABSTRACT: A simple, isocratic HPLC method based on HILIC-WAX separation, has been developed for analyzing sulfated disaccharides of glycosaminoglycans (GAGs). To our best knowledge, this is the first successful attempt using this special phase in nano-HPLC-MS analysis. Mass spectrometry was based on negative ionization, improving both sensitivity and specificity. Detection limit for most sulfated disaccharides were approximately 1?fmol; quantitation limits 10?fmol. The method was applied for glycosaminoglycan profiling of tissue samples, using surface digestion protocols. This novel combination provides sufficient sensitivity for GAG disaccharide analysis, which was first performed using prostate cancer tissue microarrays. Preliminary results show that GAG analysis may be useful for identifying cancer related changes in small amounts of tissue samples (ca. 10??g).

SUBMITTER: Turiak L 

PROVIDER: S-EPMC6590710 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature

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Sensitive method for glycosaminoglycan analysis of tissue sections.

Turiák Lilla L   Tóth Gábor G   Ozohanics Oliver O   Révész Ágnes Á   Ács András A   Vékey Károly K   Zaia Joseph J   Drahos László L  

Journal of chromatography. A 20180225


A simple, isocratic HPLC method based on HILIC-WAX separation, has been developed for analyzing sulfated disaccharides of glycosaminoglycans (GAGs). To our best knowledge, this is the first successful attempt using this special phase in nano-HPLC-MS analysis. Mass spectrometry was based on negative ionization, improving both sensitivity and specificity. Detection limit for most sulfated disaccharides were approximately 1 fmol; quantitation limits 10 fmol. The method was applied for glycosaminogl  ...[more]

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