Ontology highlight
ABSTRACT:
SUBMITTER: Turiak L
PROVIDER: S-EPMC6590710 | biostudies-literature | 2018 Apr
REPOSITORIES: biostudies-literature
Turiák Lilla L Tóth Gábor G Ozohanics Oliver O Révész Ágnes Á Ács András A Vékey Károly K Zaia Joseph J Drahos László L
Journal of chromatography. A 20180225
A simple, isocratic HPLC method based on HILIC-WAX separation, has been developed for analyzing sulfated disaccharides of glycosaminoglycans (GAGs). To our best knowledge, this is the first successful attempt using this special phase in nano-HPLC-MS analysis. Mass spectrometry was based on negative ionization, improving both sensitivity and specificity. Detection limit for most sulfated disaccharides were approximately 1 fmol; quantitation limits 10 fmol. The method was applied for glycosaminogl ...[more]