Probing the active fraction of soil microbiomes using BONCAT-FACS.
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ABSTRACT:
SUBMITTER: Couradeau E
PROVIDER: S-EPMC6591230 | biostudies-literature | 2019 Jun
REPOSITORIES: biostudies-literature
Publications
Probing the active fraction of soil microbiomes using BONCAT-FACS.
Couradeau Estelle E Sasse Joelle J Goudeau Danielle D Nath Nandita N Hazen Terry C TC Bowen Ben P BP Chakraborty Romy R Malmstrom Rex R RR Northen Trent R TR
Nature communications 20190624 1
The ability to link soil microbial diversity to soil processes requires technologies that differentiate active microbes from extracellular DNA and dormant cells. Here, we use BONCAT (bioorthogonal non-canonical amino acid tagging) to measure translationally active cells in soils. We compare the active population of two soil depths from Oak Ridge (Tennessee, USA) and find that a maximum of 25-70% of the extractable cells are active. Analysis of 16S rRNA sequences from BONCAT-positive cells recove ...[more]
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