Dataset Information

Revealing higher than expected diversity of Harpacticoida (Crustacea:Copepoda) in the North Sea using MALDI-TOF MS and molecular barcoding.

ABSTRACT: The North Sea is one of the most extensively studied marine regions of the world. Hence, large amounts of molecular data for species identification are available in public repositories, and expectations to find numerous new species in this well-known region are rather low. However, molecular reference data for harpacticoid copepods from this area in particular but also for this group in general is scarce. By assessing COI barcodes and MALDI-TOF mass spectra for this group of small crustaceans, it was discovered that there is a huge unknown diversity in this area. In total, COI sequences for 548 specimens from 115 species of harpacticoid copepods are presented. Over 19% of these were new to science and ten MOTUs were found to be part of cryptic species complexes. MALDI-TOF mass spectra were assessed for 622 specimens from 75 species. Because results were in concordance with species delimitation by COI barcoding and also enabled recognition of possible cryptic species, the discriminative power of this technique for biodiversity assessments is highlighted. Findings imply, species diversity in this group may be largely underestimated and total species number can be expected to be much higher than previously assumed.

SUBMITTER: Rossel S

PROVIDER: S-EPMC6591307 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Revealing higher than expected diversity of Harpacticoida (Crustacea:Copepoda) in the North Sea using MALDI-TOF MS and molecular barcoding.

Rossel S S Martínez Arbizu P P

Scientific reports 20190624 1

The North Sea is one of the most extensively studied marine regions of the world. Hence, large amounts of molecular data for species identification are available in public repositories, and expectations to find numerous new species in this well-known region are rather low. However, molecular reference data for harpacticoid copepods from this area in particular but also for this group in general is scarce. By assessing COI barcodes and MALDI-TOF mass spectra for this group of small crustaceans, i ...[more]

PMID: 31235850

Similar Datasets

Project description:A new species of the genus Stygepactophanes Moeschler & Rouch, 1984 (Copepoda, Harpacticoida, Canthocamptidae) is established to accommodate a small canthocamptid population collected from a spring system in the "Parc du Mercantour", Var catchment, southern France. The population analysed in the present study is defined by a set of morphological characters of the female, namely a very large maxilliped, a rudimentary mandibular palp, P1 with 3-segmented exopod and 2-segmented endopod, a falcate terminal claw of the P1 endopod, dorsal seta of caudal rami inserted on the inner margin, and anal operculum not overreaching the insertion of the caudal rami, thus supporting its assignment into the genus Stygepactophanes. The new species Stygepactophanesoccitanus shows marked differences with the nominotypical species of the genus that was originally described by monotypy with the species Stygepactophanesjurassicus Moeschler & Rouch, 1984. The main diagnostic traits of S.jurassicus are the absence of the P5 and a falcate outer terminal claw of P1 endopod. Stygepactophanesjurassicus also shows a reduced armature of the antennal exopod, bearing one seta, 1-segmented P2-P4 endopods, a reduced armature of P2-P4 exopodal segments 3 (3,4,4 armature elements, respectively), P6 bearing only one long seta, a rounded short and smooth anal operculum. Conversely the female of S.occitanus Galassi & Fiers, sp. n. has a well-developed P5, with rudimentary intercoxal sclerite, together with a falcate outer terminal claw of P1 endopod, antennal exopod bearing two elements, P4 endopod 1-segmented versus 2-segmented in P2-P3, P2-P4 exopodal segment 3 with five armature elements, P6 with three setae of different lengths, rounded anal operculum, bearing 3-4 strong spinules. According to our present knowledge, S.occitanus Galassi & Fiers, sp. n. is assigned to the genus Stygepactophanes as the most conservative solution, waiting for the male to be discovered. The genus Stygepactophanes represents a distinct lineage within the harpacticoid family Canthocamptidae that colonised southern European groundwater, the genus being known only from the saturated karst in Switzerland and a fissured saturated aquifer in southern France. Both species of the genus are stygobites and narrow endemics, the nominotypical species being known from the type locality Source de la Doux in Délemont (Switzerland), and S.occitanus Galassi & Fiers, sp. n. described herein from a spring system of the Var catchment (France).

Dataset Information

Revealing higher than expected diversity of Harpacticoida (Crustacea:Copepoda) in the North Sea using MALDI-TOF MS and molecular barcoding.

Publications

Revealing higher than expected diversity of Harpacticoida (Crustacea:Copepoda) in the North Sea using MALDI-TOF MS and molecular barcoding.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets