Ontology highlight
ABSTRACT:
SUBMITTER: Deng Y
PROVIDER: S-EPMC6591319 | biostudies-literature | 2019 Jun
REPOSITORIES: biostudies-literature
Deng Yibing Y Wu Tao T Wang Mengdi M Shi Shengchao S Yuan Guodong G Li Xi X Chong Hanchung H Wu Bin B Zheng Peng P
Nature communications 20190624 1
The recent development of chemical and bio-conjugation techniques allows for the engineering of various protein polymers. However, most of the polymerization process is difficult to control. To meet this challenge, we develop an enzymatic procedure to build polyprotein using the combination of a strict protein ligase OaAEP1 (Oldenlandia affinis asparaginyl endopeptidases 1) and a protease TEV (tobacco etch virus). We firstly demonstrate the use of OaAEP1-alone to build a sequence-uncontrolled ub ...[more]