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Integrative modeling of the HIV-1 ribonucleoprotein complex.

ABSTRACT: A coarse-grain computational method integrates biophysical and structural data to generate models of HIV-1 genomic RNA, nucleocapsid and integrase condensed into a mature ribonucleoprotein complex. Several hypotheses for the initial structure of the genomic RNA and oligomeric state of integrase are tested. In these models, integrase interaction captures features of the relative distribution of gRNA in the immature virion and increases the size of the RNP globule, and exclusion of nucleocapsid from regions with RNA secondary structure drives an asymmetric placement of the dimerized 5'UTR at the surface of the RNP globule.

SUBMITTER: Goodsell DS

PROVIDER: S-EPMC6592547 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature

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Integrative modeling of the HIV-1 ribonucleoprotein complex.

Goodsell David S DS Jewett Andrew A Olson Arthur J AJ Forli Stefano S

PLoS computational biology 20190613 6

A coarse-grain computational method integrates biophysical and structural data to generate models of HIV-1 genomic RNA, nucleocapsid and integrase condensed into a mature ribonucleoprotein complex. Several hypotheses for the initial structure of the genomic RNA and oligomeric state of integrase are tested. In these models, integrase interaction captures features of the relative distribution of gRNA in the immature virion and increases the size of the RNP globule, and exclusion of nucleocapsid fr ...[more]

PMID: 31194731

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