Thermal constraints on in vivo optogenetic manipulations.
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ABSTRACT:
SUBMITTER: Owen SF
PROVIDER: S-EPMC6592769 | biostudies-literature | 2019 Jul
REPOSITORIES: biostudies-literature
Publications
Thermal constraints on in vivo optogenetic manipulations.
Owen Scott F SF Liu Max H MH Kreitzer Anatol C AC
Nature neuroscience 20190617 7
A key assumption of optogenetics is that light only affects opsin-expressing neurons. However, illumination invariably heats tissue, and many physiological processes are temperature-sensitive. Commonly used illumination protocols increased the temperature by 0.2-2 °C and suppressed spiking in multiple brain regions. In the striatum, light delivery activated an inwardly rectifying potassium conductance and biased rotational behavior. Thus, careful consideration of light-delivery parameters is req ...[more]
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