Enhancing the functionality of a microscale bioreactor system as an industrial process development tool for mammalian perfusion culture.

ABSTRACT: Without a scale-down model for perfusion, high resource demand makes cell line screening or process development challenging, therefore, potentially successful cell lines or perfusion processes are unrealized and their ability untapped. We present here the refunctioning of a high-capacity microscale system that is typically used in fed-batch process development to allow perfusion operation utilizing in situ gravity settling and automated sampling. In this low resource setting, which involved routine perturbations in mixing, pH and dissolved oxygen concentrations, the specific productivity and the maximum cell concentration were higher than 3.0 × 10⁶  mg/cell/day and 7 × 10 ⁷ cells/ml, respectively, across replicate microscale perfusion runs conducted at one vessel volu

SUBMITTER: Sewell DJ 

PROVIDER: S-EPMC6593443 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature