Ontology highlight
ABSTRACT:
SUBMITTER: Khong D
PROVIDER: S-EPMC6594066 | biostudies-literature | 2018 Jan
REPOSITORIES: biostudies-literature
Khong Danika D Li Matthew M Singleton Amy A Chin Ling-Yee LY Parekkadan Biju B
Biomedical microdevices 20180120 1
There is an emerging need to process, expand, and even genetically engineer hematopoietic stem and progenitor cells (HSPCs) prior to administration for blood reconstitution therapy. A closed-system and automated solution for ex vivo HSC processing can improve adoption and standardize processing techniques. Here, we report a recirculating flow bioreactor where HSCs are stabilized and enriched for short-term processing by indirect fibroblast feeder coculture. Mouse 3 T3 fibroblasts were seeded on ...[more]