Dataset Information

LncRNA FEZF1-AS1 Promotes TGF-?2-Mediated Proliferation and Migration in Human Lens Epithelial Cells SRA01/04.

ABSTRACT: Posterior capsule opacification (PCO) is a common complication after cataract surgery attributed to the proliferation and migration of postoperative residual lens epithelial cells (LECs). The long noncoding RNA (lncRNA) FEZ family zinc finger 1 antisense RNA 1 (FEZF1-AS1) promotes the proliferation and migration of multiple types of cancer cells. Here, we discovered that FEZF1-AS1 is markedly upregulated in TGF-?2-treated SRA01/04?cells. In addition, the proliferation and migration of SRA01/04?cells were enhanced following TGF-?2 treatment. FEZF1-AS1 knockdown inhibited the TGF-?2-induced proliferation and migration of SRA01/04?cells. Accordingly, FEZF1-AS1 overexpression promoted the TGF-?2-induced proliferation and migration of SRA01/04?cells. Finally, FEZF1-AS1 upregulated TGF-?2-induced SRA01/04?cell proliferation and migration via boosting FEZF1 protein levels. Our findings indicate that the dysregulation of FEZF1-AS1 participates in the TGF-?2-induced proliferation and migration of human lens epithelial cells (HLECs), which might be achieved, at least in part, through the induction of FEZF1 expression.

SUBMITTER: Wang Y

PROVIDER: S-EPMC6594282 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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LncRNA FEZF1-AS1 Promotes TGF-<i>β</i>2-Mediated Proliferation and Migration in Human Lens Epithelial Cells SRA01/04.

Wang Yong Y Chen Lili L Gu Yonghui Y Wang Ying Y Yuan You Y Zhu Qiujian Q Bi Mingchao M Gu Shuyan S

Journal of ophthalmology 20190612

Posterior capsule opacification (PCO) is a common complication after cataract surgery attributed to the proliferation and migration of postoperative residual lens epithelial cells (LECs). The long noncoding RNA (lncRNA) FEZ family zinc finger 1 antisense RNA 1 (FEZF1-AS1) promotes the proliferation and migration of multiple types of cancer cells. Here, we discovered that FEZF1-AS1 is markedly upregulated in TGF-<i>β</i>2-treated SRA01/04 cells. In addition, the proliferation and migration of SRA ...[more]

PMID: 31281667

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Project description:BackgroundLong non-coding RNAs (lncRNAs) are pervasively transcribed in genome and emerging as a new player in tumorigenesis due to their functions in transcriptional, posttranscriptional and epigenetic mechanisms of gene regulation. As the most frequent malignancy and the foremost source of cancer mortality, lung cancer is a heterogeneous disorder. The most common type of lung cancer is Non-small cell lung cancer (NSCLC), occupying 85% of the total cases, and the main subtypes of NSCLC include lung adenocarcinoma (LAD), large cell carcinoma (LCC), and lung squamous cell carcinoma (LSCC). Recently, numerous lncRNAs have been reported to be strongly linked to NSCLC. In the present study, we found that a new lncRNA CBR3-AS1 is highly expressed in lung cancer. In addition, we also examined the expression of lncRNA CBR3-AS1 in 60 of LADs, 40 of LCCs and 40 of LSCCs patient samples, finding that CBR3-AS1 was specificity highly expressed in LAD cancer tissues. Mechanically, we discovered that CBR3-AS1 could regulate the proliferation, migration and invasion of LAD cells through targeting Wnt/?-catenin signaling.MethodsReal-time PCR, RNA-pulldown, RIP, western blotting, lentivirus transfection, luciferase reporter assays, cell proliferation assays, colony formation assays, wound healing scratch assays and transwell assays were employed to examine the relationship between lncRNA CBR3-AS1 and its regulation of Wnt/?-catenin signaling in LAD cells.ResultsLncRNA CBR3-AS1 is highly-expressed in LAD and cell lines. LncRNA CBR3-AS1 shows physical association with ?-catenin. CBR3-AS1 could facilitate Wnt/?-catenin signaling activation thought promoting nuclear localization of ?-catenin. CBR3-AS1 promotes LAD cell proliferation, migration and invasion by targeting Wnt/?-catenin signaling.ConclusionIt can be found that a new functional lncRNA CBR3-AS1 could promote nuclear localization of ?-catenin so as to facilitate Wnt/?-catenin signaling activation and regulate the proliferation, migration and invasion of LAD cells.

Dataset Information

LncRNA FEZF1-AS1 Promotes TGF-?2-Mediated Proliferation and Migration in Human Lens Epithelial Cells SRA01/04.

Publications

LncRNA FEZF1-AS1 Promotes TGF-<i>β</i>2-Mediated Proliferation and Migration in Human Lens Epithelial Cells SRA01/04.

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