Ontology highlight
ABSTRACT:
SUBMITTER: Li J
PROVIDER: S-EPMC6594443 | biostudies-literature | 2019 Feb
REPOSITORIES: biostudies-literature
Molecular biology of the cell 20181219 4
In mammalian cells, the Golgi reassembly stacking protein of 65 kDa (GRASP65) has been implicated in both Golgi stacking and ribbon linking by forming trans-oligomers. To better understand its function and regulation, we used biochemical methods to identify the DnaJ homolog subfamily A member 1 (DjA1) as a novel GRASP65-binding protein. In cells, depletion of DjA1 resulted in Golgi fragmentation, short and improperly aligned cisternae, and delayed Golgi reassembly after nocodazole washout. In vi ...[more]