A split fluorescent reporter with rapid and reversible complementation.

ABSTRACT: Interactions between proteins play an essential role in metabolic and signaling pathways, cellular processes and organismal systems. We report the development of splitFAST, a fluorescence complementation system for the visualization of transient protein-protein interactions in living cells. Engineered from the fluorogenic reporter FAST (Fluorescence-Activating and absorption-Shifting Tag), which specifically and reversibly binds fluorogenic hydroxybenzylidene rhodanine (HBR) analogs, splitFAST displays rapid and reversible complementation, allowing the real-time visualization of both the formation and the dissociation of a protein assembly.

SUBMITTER: Tebo AG 

PROVIDER: S-EPMC6597557 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature