Nanosecond pulsed electric field induced proliferation and differentiation of osteoblasts and myoblasts.

ABSTRACT: Low-intensity electric fields can induce changes in cell differentiation and cytoskeletal stresses that facilitate manipulation of osteoblasts and mesenchymal stem cells; however, the application times (tens of minutes) are of the order of physiological mechanisms, which can complicate treatment consistency. Intense nanosecond pulsed electric fields (nsPEFs) can overcome these challenges by inducing similar stresses on shorter timescales while additionally inducing plasma membrane nanoporation, ion transport and intracellular structure manipulation. This paper shows that treating myoblasts and osteoblasts with five 300 ns PEFs with intensities from 1.5 to 25 kV cm^-1 increased proliferation and differentiation. While nsPEFs above 5 kV cm^-1 decreased myoblast population

SUBMITTER: Vadlamani RA 

PROVIDER: S-EPMC6597781 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature