Ontology highlight
ABSTRACT:
SUBMITTER: Velagapudi SP
PROVIDER: S-EPMC6598432 | biostudies-literature | 2019 Jun
REPOSITORIES: biostudies-literature
Bioorganic & medicinal chemistry letters 20190402 12
Methods to identify RNAs bound by small molecules in cells are sparse. Herein, an advance to identify the direct RNA targets of small molecules in cells is described. The approach, dubbed Chemical Cross-Linking and Isolation by Pull-down to Map Small Molecule-RNA Binding Sites (Chem-CLIP-Map-Seq), appends a cross-linker and a purification tag onto a small molecule. In cells, the compound binds to RNA and undergoes a proximity-based reaction. The cross-linked RNA is purified and then amplified us ...[more]