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MiRkwood: a tool for the reliable identification of microRNAs in plant genomes.


ABSTRACT: BACKGROUND:MicroRNAs (miRNAs) play crucial roles in post-transcriptional regulation of eukaryotic gene expression and are involved in many aspects of plant development. Although several prediction tools are available for metazoan genomes, the number of tools dedicated to plants is relatively limited. RESULTS:Here, we present miRkwood, a user-friendly tool for the identification of miRNAs in plant genomes using small RNA sequencing data. Deep-sequencing data of Argonaute associated small RNAs showed that miRkwood is able to identify a large diversity of plant miRNAs and limits false positive predictions. Moreover, it outperforms current tools such as ShortStack and contrary to ShortStack, miRkwood provides a quality score allowing users to rank miRNA predictions. CONCLUSION:miRkwood is a very efficient tool for the annotation of miRNAs in plant genomes. It is available as a web server, as a standalone version, as a docker image and as a Galaxy tool: http://bioinfo.cristal.univ-lille.fr/mirkwood.

SUBMITTER: Guigon I 

PROVIDER: S-EPMC6599362 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature

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miRkwood: a tool for the reliable identification of microRNAs in plant genomes.

Guigon Isabelle I   Legrand Sylvain S   Berthelot Jean-Frédéric JF   Bini Sébastien S   Lanselle Delphine D   Benmounah Mohcen M   Touzet Hélène H  

BMC genomics 20190628 1


<h4>Background</h4>MicroRNAs (miRNAs) play crucial roles in post-transcriptional regulation of eukaryotic gene expression and are involved in many aspects of plant development. Although several prediction tools are available for metazoan genomes, the number of tools dedicated to plants is relatively limited.<h4>Results</h4>Here, we present miRkwood, a user-friendly tool for the identification of miRNAs in plant genomes using small RNA sequencing data. Deep-sequencing data of Argonaute associated  ...[more]

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