Interactions between ?-Lactoglobulin and 3,3'-Diindolylmethane in Model System.

ABSTRACT: The compound 3,3'-diindolylmethane (DIM) has a broad spectrum of anticancer activities. However, low stability and bioavailability limit its application. Elucidating interactions between DIM and ?-lactoglobulin (?-LG) may be useful for fabricating whey protein-based protecting systems. Interaction with DIM increased the diameter and absolute zeta potential value of ?-LG. UV-absorption spectra suggested that there was a complex of DIM and ?-LG. ?-LG showed enhanced fluorescence intensity by complexing with DIM with a binding constant of 6.7 × 105 M-1. Upon interaction with DIM, ?-LG was decreased in secondary structure content of helix and turn while increased in ?-sheet and unordered. FT-IR spectra and molecular docking results indicated the roles of hydrophobic interaction and hydrogen bond for the formation of DIM and ?-LG nanocomplexes. Data suggested that ?-LG may be a good vehicle for making a protein-based DIM protection and delivery system due to the tight binding of DIM to ?-LG.

SUBMITTER: Wang C 

PROVIDER: S-EPMC6600512 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature