Interactions between β-Lactoglobulin and 3,3'-Diindolylmethane in Model System.

ABSTRACT: The compound 3,3'-diindolylmethane (DIM) has a broad spectrum of anticancer activities. However, low stability and bioavailability limit its application. Elucidating interactions between DIM and β-lactoglobulin (β-LG) may be useful for fabricating whey protein-based protecting systems. Interaction with DIM increased the diameter and absolute zeta potential value of β-LG. UV-absorption spectra suggested that there was a complex of DIM and β-LG. β-LG showed enhanced fluorescence intensity by complexing with DIM with a binding constant of 6.7 × 10⁵ M^-1. Upon interaction with DIM, β-LG was decreased in secondary structure content of helix and turn while increased in β-sheet and unordered. FT-IR spectra and molecular docking results indicated the roles of hydrophobic inter

SUBMITTER: Wang C 

PROVIDER: S-EPMC6600512 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature