Ontology highlight
ABSTRACT:
SUBMITTER: Parthiban K
PROVIDER: S-EPMC6601556 | biostudies-literature | 2019 Jul
REPOSITORIES: biostudies-literature
Parthiban Kothai K Perera Rajika L RL Sattar Maheen M Huang Yanchao Y Mayle Sophie S Masters Edward E Griffiths Daniel D Surade Sachin S Leah Rachael R Dyson Michael R MR McCafferty John J
mAbs 20190609 5
The construction of large libraries in mammalian cells allows the direct screening of millions of molecular variants for binding properties in a cell type relevant for screening or production. We have created mammalian cell libraries of up to 10 million clones displaying a repertoire of IgG-formatted antibodies on the cell surface. TALE nucleases or CRISPR/Cas9 were used to direct the integration of the antibody genes into a single genomic locus, thereby rapidly achieving stable expression and t ...[more]