Dataset Information

Deciphering the genetic basis for vitamin E accumulation in leaves and grains of different barley accessions.

ABSTRACT: Tocopherols and tocotrienols, commonly referred to as vitamin E, are essential compounds in food and feed. Due to their lipophilic nature they protect biomembranes by preventing the propagation of lipid-peroxidation especially during oxidative stress. Since their synthesis is restricted to photosynthetic organisms, plant-derived products are the major source of natural vitamin E. In the present study the genetic basis for high vitamin E accumulation in leaves and grains of different barley (Hordeum vulgare L.) accessions was uncovered. A genome wide association study (GWAS) allowed the identification of two genes located on chromosome 7H, homogentisate phytyltransferase (HPT-7H) and homogentisate geranylgeranyltransferase (HGGT) that code for key enzymes controlling the accumulation of tocopherols in leaves and tocotrienols in grains, respectively. Transcript profiling showed a correlation between HPT-7H expression and vitamin E content in leaves. Allele sequencing allowed to decipher the allelic variation of HPT-7H and HGGT genes corresponding to high and low vitamin E contents in the respective tissues. Using the obtained sequence information molecular markers have been developed which can be used to assist smart breeding of high vitamin E barley varieties. This will facilitate the selection of genotypes more tolerant to oxidative stress and producing high-quality grains.

SUBMITTER: Schuy C

PROVIDER: S-EPMC6602966 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature

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Deciphering the genetic basis for vitamin E accumulation in leaves and grains of different barley accessions.

Schuy Christian C Groth Jennifer J Ammon Alexandra A Eydam Julia J Baier Steffen S Schweizer Günther G Hanemann Anja A Herz Markus M Voll Lars M LM Sonnewald Uwe U

Scientific reports 20190701 1

Tocopherols and tocotrienols, commonly referred to as vitamin E, are essential compounds in food and feed. Due to their lipophilic nature they protect biomembranes by preventing the propagation of lipid-peroxidation especially during oxidative stress. Since their synthesis is restricted to photosynthetic organisms, plant-derived products are the major source of natural vitamin E. In the present study the genetic basis for high vitamin E accumulation in leaves and grains of different barley (Hord ...[more]

PMID: 31263124

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Dataset Information

Deciphering the genetic basis for vitamin E accumulation in leaves and grains of different barley accessions.

Publications

Deciphering the genetic basis for vitamin E accumulation in leaves and grains of different barley accessions.

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