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Automated high-throughput high-content autophagy and mitophagy analysis platform.

ABSTRACT: Autophagic processes play a central role in cellular homeostasis. In pathological conditions, the flow of autophagy can be affected at multiple and distinct steps of the pathway. Current analyses tools do not deliver the required detail for dissecting pathway intermediates. The development of new tools to analyze autophagic processes qualitatively and quantitatively in a more straightforward manner is required. Defining all autophagy pathway intermediates in a high-throughput manner is technologically challenging and has not been addressed yet. Here, we overcome those requirements and limitations by the developed of stable autophagy and mitophagy reporter-iPSC and the establishment of a novel high-throughput phenotyping platform utilizing automated high-content image analysis to assess autophagy and mitophagy pathway intermediates.

SUBMITTER: Arias-Fuenzalida J

PROVIDER: S-EPMC6603000 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature

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Automated high-throughput high-content autophagy and mitophagy analysis platform.

Arias-Fuenzalida Jonathan J Jarazo Javier J Walter Jonas J Gomez-Giro Gemma G Forster Julia I JI Krueger Rejko R Antony Paul M A PMA Schwamborn Jens C JC

Scientific reports 20190701 1

Autophagic processes play a central role in cellular homeostasis. In pathological conditions, the flow of autophagy can be affected at multiple and distinct steps of the pathway. Current analyses tools do not deliver the required detail for dissecting pathway intermediates. The development of new tools to analyze autophagic processes qualitatively and quantitatively in a more straightforward manner is required. Defining all autophagy pathway intermediates in a high-throughput manner is technolog ...[more]

PMID: 31263238

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